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PUR4 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PUR4 Antibody  (N-term) AP4751a
    Western blot analysis of PUR4 Antibody (N-term) (Cat. #AP4751a) in HepG2 cell line lysates (35ug/lane). PUR4 (arrow) was detected using the purified Pab.
    detail
  • WB - PUR4 Antibody  (N-term) AP4751a
    All lanes : Anti-PUR4 Antibody (N-term) at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 145 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - PUR4 Antibody  (N-term) AP4751a
    PUR4 Antibody (N-term) (Cat. #AP4751a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUR4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - PUR4 Antibody  (N-term) AP4751a
    PUR4 Antibody (N-term) (Cat. #AP4751a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O15067
Other Accession Q5SUR0
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 144734 Da
Antigen Region 184-213 aa
Additional Information
Gene ID 5198
Other Names Phosphoribosylformylglycinamidine synthase, FGAM synthase, FGAMS, Formylglycinamide ribonucleotide amidotransferase, FGAR amidotransferase, FGAR-AT, Formylglycinamide ribotide amidotransferase, PFAS, KIAA0361
Target/Specificity This PUR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-213 amino acids from the N-terminal region of human PUR4.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPUR4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PFAS
Synonyms KIAA0361
Function Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 365.00
$ 140.00
Cat# AP4751a
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