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BHLHB5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BHLHB5 Antibody (Center) AP4777C
    Western blot analysis of BHLHB5 Antibody (Center) (Cat. #AP4777c) in HL-60 cell line lysates (35ug/lane). BHLHB5 (arrow) was detected using the purified Pab.
  • IHC-P - BHLHB5 Antibody (Center) AP4777C
    BHLHB5 Antibody (Center) (Cat. #AP4777c) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - BHLHB5 Antibody (Center) AP4777C
    BHLHB5 Antibody (Center) (Cat. #AP4777c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q8NFJ8
Other Accession Q8C6A8, Q71T09
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 236-264 aa
Additional Information
Other Names Class E basic helix-loop-helix protein 22, bHLHe22, Class B basic helix-loop-helix protein 5, bHLHb5, Trinucleotide repeat-containing gene 20 protein, BHLHE22, BHLHB5, TNRC20
Target/Specificity This BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human BHLHB5.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBHLHB5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BHLHE22
Synonyms BHLHB5, TNRC20
Function Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA (By similarity). In the brain, may function as an area-specific transcription factor that regulates the postmitotic acquisition of area identities and elucidate the genetic hierarchy between progenitors and postmitotic neurons driving neocortical arealization. May be required for the survival of a specific population of inhibitory neurons in the superficial laminae of the spinal chord dorsal horn that may regulate pruritis. Seems to play a crucial role in the retinogenesis, in the specification of amacrine and bipolar subtypes. Forms with PRDM8 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation.
Cellular Location Nucleus.
Tissue Location Brain-specific, with the highest expression in the cerebellum. EMBL; AF504925; AAM28881.1; -; Genomic_DNA EMBL; BK000273; DAA01053.1; -; mRNA CCDS; CCDS6179.1; - RefSeq; NP_689627.1; NM_152414.4 UniGene; Hs.388788; - UniGene; Hs.591870; - UniGene; Hs.745052; - ProteinModelPortal; Q8NFJ8; - SMR; Q8NFJ8; - STRING; 9606.ENSP00000318799; - iPTMnet; Q8NFJ8; - PhosphoSitePlus; Q8NFJ8; - BioMuta; BHLHE22; - DMDM; 74751284; - PaxDb; Q8NFJ8; - PeptideAtlas; Q8NFJ8; - PRIDE; Q8NFJ8; - ProteomicsDB; 73318; - DNASU; 27319; - Ensembl; ENST00000321870; ENSP00000318799; ENSG00000180828 GeneID; 27319; - KEGG; hsa:27319; - UCSC; uc003xvi.4; human CTD; 27319; - DisGeNET; 27319; - EuPathDB; HostDB:ENSG00000180828.2; - GeneCards; BHLHE22; - H-InvDB; HIX0007544; - HGNC; HGNC:11963; BHLHE22 HPA; HPA064128; - HPA; HPA064872; - HPA; HPA067500; - MIM; 613483; gene neXtProt; NX_Q8NFJ8; - OpenTargets; ENSG00000180828; - PharmGKB; PA36650; - eggNOG; KOG3898; Eukaryota eggNOG; ENOG410ZDH5; LUCA GeneTree; ENSGT00530000063239; - InParanoid; Q8NFJ8; - KO; K09086; - OMA; CKQCTDK; - OrthoDB; EOG091G129L; - PhylomeDB; Q8NFJ8; - TreeFam; TF322733; - GenomeRNAi; 27319; - PRO; PR:Q8NFJ8; - Proteomes; UP000005640; Chromosome 8 Bgee; ENSG00000180828; - Genevisible; Q8NFJ8; HS GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell GO; GO:0046983; F:protein dimerization activity; IEA:InterPro GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0022008; P:neurogenesis; IEA:InterPro GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW CDD; cd00083; HLH; 1 Gene3D; 4.10.280.10; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR032654; Bhlhe22 InterPro; IPR036638; HLH_DNA-bd_sf PANTHER; PTHR19290:SF52; PTHR19290:SF52; 1 Pfam; PF00010; HLH; 1 SMART; SM00353; HLH; 1 SUPFAM; SSF47459; SSF47459; 1 PROSITE; PS50888; BHLH; 1 2: Evidence at transcript level; Complete proteome; Neurogenesis; Nucleus; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation CHAIN 1 381 Class E basic helix-loop-helix protein 22 /FTId=PRO_0000274285 DOMAIN 242 296 bHLH. {ECO:0000255|PROSITE- ProRule:PRU00981} COMPBIAS 56 60 Poly-Ser COMPBIAS 72 225 Gly-rich COMPBIAS 208 234 Ser-rich COMPBIAS 315 357 Ala-rich VARIANT 28 28 S -> A (in dbSNP:rs7016250) /FTId=VAR_061255 SEQUENCE 381 AA; 36997 MW; 7B17A9BD5C7F8569 CRC64; MERGMHLGAA AAGEDDLFLH KSLSASTSKR LEAAFRSTPP GMDLSLAPPP RERPASSSSS PLGCFEPADP EGAGLLLPPP GGGGGGSAGS GGGGGGGVGV PGLLVGSAGV GGDPSLSSLP AGAALCLKYG ESASRGSVAE SSGGEQSPDD DSDGRCELVL RAGVADPRAS PGAGGGGAKA AEGCSNAHLH GGASVPPGGL GGGGGGGSSS GSSGGGGGSG SGSGGSSSSS SSSSKKSKEQ KALRLNINAR ERRRMHDLND ALDELRAVIP YAHSPSVRKL SKIATLLLAK NYILMQAQAL EEMRRLVAYL NQGQAISAAS LPSSAAAAAA AAALHPALGA YEQAAGYPFS AGLPPAASCP EKCALFNSVS SSLCKQCTEK P
Research Areas
Citations (0)

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Background

BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.

References

Stevens, J.D., et al. Differentiation 76(9):1006-1022(2008)
McLellan, A.S., et al. Mech. Dev. 119 SUPPL 1, S285-S291 (2002)

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Cat# AP4777C
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