|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8C6A8, Q71T09|
|Calculated MW||36997 Da|
|Antigen Region||236-264 aa|
|Other Names||Class E basic helix-loop-helix protein 22, bHLHe22, Class B basic helix-loop-helix protein 5, bHLHb5, Trinucleotide repeat-containing gene 20 protein, BHLHE22, BHLHB5, TNRC20|
|Target/Specificity||This BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human BHLHB5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BHLHB5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA (By similarity). In the brain, may function as an area-specific transcription factor that regulates the postmitotic acquisition of area identities and elucidate the genetic hierarchy between progenitors and postmitotic neurons driving neocortical arealization. May be required for the survival of a specific population of inhibitory neurons in the superficial laminae of the spinal chord dorsal horn that may regulate pruritis. Seems to play a crucial role in the retinogenesis, in the specification of amacrine and bipolar subtypes. Forms with PRDM8 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation.|
|Tissue Location||Brain-specific, with the highest expression in the cerebellum.|
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Provided below are standard protocols that you may find useful for product applications.
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
Stevens, J.D., et al. Differentiation 76(9):1006-1022(2008)
McLellan, A.S., et al. Mech. Dev. 119 SUPPL 1, S285-S291 (2002)
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