|Application ||WB, IHC-P, FC, E|
|Calculated MW||195459 Da|
|Antigen Region||1570-1600 aa|
|Other Names||Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1|
|Target/Specificity||This ZO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1570-1600 amino acids from the C-terminal region of human ZO1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZO1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.|
|Cellular Location||Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells|
|Tissue Location||The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules|
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Provided below are standard protocols that you may find useful for product applications.
ZO1 encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions.
Ohira, M., et al. Int. J. Mol. Med. 24(6):829-835(2009)
Hirakawa, H., et al. Int. J. Oncol. 35(6):1271-1276(2009)
Meerschaert, K., et al. Cell. Mol. Life Sci. 66(24):3951-3966(2009)
Kirschner, N., et al. Am. J. Pathol. 175(3):1095-1106(2009)
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