|Application ||WB, IHC-P, FC, E|
|Calculated MW||75357 Da|
|Antigen Region||63-91 aa|
|Other Names||Mitotic interactor and substrate of PLK1, Mitotic spindle positioning protein, MISP, C19orf21|
|Target/Specificity||This C19orf21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-91 amino acids from the N-terminal region of human C19orf21.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||C19orf21 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus.|
|Cellular Location||Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Predominantly localizes to cortical actin structures during interphase and mitosis Present in retraction fibers, which are formed at former adhesion sites during mitosis, and at spicular membrane protrusions in re- attaching cytokinetic cells. Partially colocalizes with cytoplasmic F-actin. Not detected at microtubules at interphase, nor at spindle during mitosis|
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Provided below are standard protocols that you may find useful for product applications.
The exact function of C19orf21 remains unknown.
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Kim, J.E., et al. J. Proteome Res. 4(4):1339-1346(2005)
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