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BLM Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BLM Antibody (C-term) AP4860b
    Western blot analysis of BLM Antibody (C-term) (Cat. #AP4860b) in HL-60 cell line lysates (35ug/lane). BLM (arrow) was detected using the purified Pab.
  • FC - BLM Antibody (C-term) AP4860b
    BLM Antibody (C-term) (Cat. #AP4860b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54132
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 159000 Da
Antigen Region 1054-1082 aa
Additional Information
Gene ID 641
Other Names Bloom syndrome protein, DNA helicase, RecQ-like type 2, RecQ2, RecQ protein-like 3, BLM, RECQ2, RECQL3
Target/Specificity This BLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1054-1082 amino acids from the C-terminal region of human BLM.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBLM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BLM
Synonyms RECQ2, RECQL3
Function Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Cellular Location Nucleus. Note=Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner
Citations (0)

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BLM is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities.


Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Frank, B., et al. Carcinogenesis 31(3):442-445(2010)
Ouyang, K.J., et al. PLoS Biol. 7 (12), E1000252 (2009)

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$ 295.00
$ 99.00
Cat# AP4860b
(40 western blots)
Availability: In Stock
Bulk Size
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