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ARHGAP25 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARHGAP25 Antibody (Center) AP4865c
    Anti-ARHGAP25 Antibody (Center) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ARHGAP25 Antibody (Center) AP4865c
    ARHGAP25 Antibody (Center) (Cat. #AP4865c) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGAP25 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P42331
Other Accession Q8BYW1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52-74 KDa
Antigen Region 193-219 aa
Additional Information
Gene ID 9938
Other Names Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25, ARHGAP25, KIAA0053
Target/Specificity This ARHGAP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-219 amino acids from the Central region of human ARHGAP25.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARHGAP25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARHGAP25
Synonyms KIAA0053
Function GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Research Areas
Citations (0)
citation

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Background

ARHGAP25 encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration.

References

Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010)
Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)
Katoh, M., et al. Int. J. Mol. Med. 14(2):333-338(2004)

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$ 182.50
$ 70.00
Cat# AP4865c
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