|Application ||WB, IHC-P, IF, E|
|Calculated MW||85659 Da|
|Antigen Region||713-742 aa|
|Other Names||Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL|
|Target/Specificity||This NR3C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 713-742 amino acids from the C-terminal region of human NR3C1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NR3C1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression.|
|Cellular Location||Cytoplasm. Mitochondrion. Nucleus Note=Cytoplasmic in the absence of ligand, nuclear after ligand- binding|
|Tissue Location||Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.|
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Provided below are standard protocols that you may find useful for product applications.
NR3C1 is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance.
Geelhoed, M.J., et al. BMC Med. Genet. 11, 39 (2010)
Szilagyi, K., et al. Neuro Endocrinol. Lett. 30(5):629-636(2009)
Tian, S., et al. Biochem. J. 367 (PT 3), 907-911 (2002)
Itoh, M., et al. Mol. Endocrinol. 16(10):2382-2392(2002)
Wang, Z., et al. J. Biol. Chem. 277(29):26573-26580(2002)
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