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HIF1A AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP4879d | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP4879d-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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HIF1A Antibody - Product info | |
| Application | WB, IHC, FC
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| Primary Accession | Q16665 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 92670 Da |
HIF1A Antibody - Additional info | |
| Gene ID 3091 | |
| Other Names HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; Member of PAS protein 1; PAS domain-containing protein 8 | |
| Target/Specificity This HIF1A antibody is generated from rabbits immunized with HIF1A recombinant protein. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by protein affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions HIF1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
HIF1A Antibody - Protein Information | |
| Name HIF1A | |
| Synonyms BHLHE78, MOP1, PASD8 | |
| Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia | |
| Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in normoxia, nuclear translocation in response to hypoxia Colocalizes with SUMO1 in the nucleus, under hypoxia | |
| Tissue Location Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors | |
HIF1A Antibody - Related products
AM1934a: HIF1A Antibody (ascites)
AP3707a: Hydroxy-HIF1A- P564 Antibody
AP4776a: HIF1A Antibody (N-term)
AP4776b: HIF1A Antibody (C-term)
AP7759b: HIF1Alpha Antibody (C-term)
AP7759c: HIF1Alpha Antibody (Center)
RI12452: HIF1A predesign siRNA
BP3707a: Hydroxy-HIF1A-P564 Antibody Blocking Peptide
BP4776a: HIF1A Antibody (N-term) Blocking Peptide
BP4776b: HIF1A Antibody (C-term) Blocking Peptide
BP4776d: HIF1A (HyP564) Antibody Blocking Peptide
BP7759b: HIF1Alpha Antibody (C-term) Blocking Peptide
HIF1A Antibody - Application data
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Western blot analysis of HIF1A Antibody (Cat. #AP4879d) in K562 cell line lysates (35ug/lane). HIF1A (arrow) was detected using the purified Pab.
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HIF1A Antibody (Cat. #AP4879d) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF1A Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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HIF1A Antibody (Cat. #AP4879d) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HIF1A Antibody - Research Areas
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BACKGROUND
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.
REFERENCES
Lee, M.N., et al. J. Natl. Cancer Inst. 102(6):426-442(2010) Mayer, A., et al. Adv. Exp. Med. Biol. 662, 399-405 (2010) Brouwer, E., et al. Clin. Exp. Rheumatol. 27(6):945-951(2009)
