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NASP Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, WB, E |
|---|---|
| Primary Accession | P49321 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 85238 Da |
| Antigen Region | 152-180 aa |
| Gene ID | 4678 |
|---|---|
| Other Names | Nuclear autoantigenic sperm protein, NASP, NASP |
| Target/Specificity | This NASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the N-terminal region of human NASP. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NASP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NASP |
|---|---|
| Function | Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q99MD9}. Nucleus {ECO:0000250|UniProtKB:Q99MD9} |
| Tissue Location | Isoform 1 is testis- and sperm-specific. |
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Provided below are standard protocols that you may find useful for product applications.
Background
NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
References
Alekseev, O.M., et al. Reprod. Biol. Endocrinol. 7, 45 (2009)
Wang, H., et al. Nucleic Acids Res. 36(18):5763-5772(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
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