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NASP Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP4911a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP4911a-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
NASP Antibody (N-term) - Product info | |
| Application | WB, FC
|
| Primary Accession | P49321 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 85238 Da |
NASP Antibody (N-term) - Additional info | |
| Gene ID 4678 | |
| Other Names NASP; Nuclear autoantigenic sperm protein | |
| Target/Specificity This NASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 160-190 from the N-terminal region of human NASP. | |
| Dilution WB~~1:50~100WB~~1:1000 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions NASP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
NASP Antibody (N-term) - Protein Information | |
| Name NASP | |
| Function Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity) | |
| Cellular Location Cytoplasm (By similarity). Nucleus. | |
| Tissue Location Isoform 1 is testis- and sperm-specific. | |
NASP Antibody (N-term) - Related products
AP2806b: NASP Antibody (C-term)
AP4911a: NASP Antibody (N-term)
NASP Antibody (N-term) - Application data
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Western blot analysis of NASP Antibody (N-term)) (Cat. #AP4911a) in HL-60 cell line lysates (35ug/lane).NASP (arrow) was detected using the purified Pab.
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Western blot analysis of NASP (arrow) using rabbit polyclonal NASP Antibody (N-term) (Cat. #AP4911a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2) .
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NASP Antibody (N-term) (Cat. #AP4911a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NASP Antibody (N-term) - Research Areas
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BACKGROUND
NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
REFERENCES
Alekseev, O.M., et al. Reprod. Biol. Endocrinol. 7, 45 (2009) Wang, H., et al. Nucleic Acids Res. 36(18):5763-5772(2008) Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
