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NASP Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NASP Antibody (N-term) AP4911a
    All lanes : Anti-NASP Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - NASP Antibody (N-term) AP4911a
    Western blot analysis of NASP (arrow) using rabbit polyclonal NASP Antibody (N-term) (Cat. #AP4911a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2) .
  • FC - NASP Antibody (N-term) AP4911a
    NASP Antibody (N-term) (Cat. #AP4911a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49321
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85238 Da
Antigen Region 152-180 aa
Additional Information
Gene ID 4678
Other Names Nuclear autoantigenic sperm protein, NASP, NASP
Target/Specificity This NASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the N-terminal region of human NASP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNASP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity).
Cellular Location Cytoplasm. Nucleus.
Tissue Location Isoform 1 is testis- and sperm-specific.
Research Areas
Citations (0)

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NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.


Alekseev, O.M., et al. Reprod. Biol. Endocrinol. 7, 45 (2009)
Wang, H., et al. Nucleic Acids Res. 36(18):5763-5772(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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$ 295.00
$ 99.00
Cat# AP4911a
(40 western blots)
Availability: In Stock
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