|Application ||WB, FC, E|
|Calculated MW||39555 Da|
|Antigen Region||311-339 aa|
|Other Names||Centromere protein N, CENP-N, Interphase centromere complex protein 32, CENPN, C16orf60, ICEN32|
|Target/Specificity||This CENPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-339 amino acids from the C-terminal region of human CENPN.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CENPN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis|
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Provided below are standard protocols that you may find useful for product applications.
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPN is an additional factor required for centromere assembly.
Carroll, C.W., et al. Nat. Cell Biol. 11(7):896-902(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Izuta, H., et al. Genes Cells 11(6):673-684(2006)
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