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RPC8 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RPC8 Antibody (N-term) AP4936a
    Western blot analysis of RPC8 Antibody (N-term) (Cat. #AP4936a) in mouse testis tissue lysates (35ug/lane). RPC8 (arrow) was detected using the purified Pab.
  • FC - RPC8 Antibody (N-term) AP4936a
    RPC8 Antibody (N-term) (Cat. #AP4936a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9Y535
Other Accession Q9D2C6
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22918 Da
Antigen Region 5-33 aa
Additional Information
Gene ID 171568
Other Names DNA-directed RNA polymerase III subunit RPC8, RNA polymerase III subunit C8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 229 kDa subunit, RPC229, POLR3H, KIAA1665, RPC8
Target/Specificity This RPC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human RPC8.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPC8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLR3H
Synonyms KIAA1665, RPC8
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).
Cellular Location Nucleus.
Citations (0)

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Background

RPC8 is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. It is acting as nuclear and cytosolic DNA sensor involved in innate immune response. It can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

References

Greco-Stewart, V.S., et al. Virology 386(1):12-15(2009)
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004)
Hu, P., et al. Mol. Cell. Biol. 22(22):8044-8055(2002)

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$ 295.00
$ 99.00
Cat# AP4936a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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