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MFAP4 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MFAP4 Antibody (C-term) AP4940b
    Western blot analysis of lysate from human lung tissue lysate, using MFAP4 Antibody (C-term)(Cat. #AP4940b). AP4940b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
    detail
  • IHC-P - MFAP4 Antibody (C-term) AP4940b
    MFAP4 Antibody (C-term) (Cat. #AP4940b) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFAP4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - MFAP4 Antibody (C-term) AP4940b
    MFAP4 Antibody (C-term) (Cat. #AP4940b) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • WB - MFAP4 Antibody (C-term) AP4940b
    Western blot analysis of MFAP4 Antibody (C-term) (Cat. #AP4940b) in mouse lung tissue lysates (35ug/lane). MFAP4 (arrow) was detected using the purified Pab.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P55083
Other Accession Q9D1H9, P55918
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 28648 Da
Antigen Region 228-255 aa
Additional Information
Gene ID 4239
Other Names Microfibril-associated glycoprotein 4, MFAP4
Target/Specificity This MFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the C-terminal region of human MFAP4.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMFAP4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MFAP4
Function Could be involved in calcium-dependent cell adhesion or intercellular interactions. May contribute to the elastic fiber assembly and/or maintenance (PubMed:26601954).
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations (0)
citation

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Background

MFAP4 is a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region.

References

Edelman, E.A., et al. Clin. Genet. 71(6):540-550(2007)
Schlosser, A., et al. Scand. J. Immunol. 64(2):104-116(2006)
Liu, T., et al. J. Proteome Res. 4(6):2070-2080(2005)

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$ 365.00
$ 140.00
Cat# AP4940b
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