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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RRAGD Antibody (Center)   

RRAGD Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RRAGD Antibody  (Center) AP4959c
    Anti-RRAGD Antibody (Center) at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RRAGD Antibody  (Center) AP4959c
    Western blot analysis of RRAGD Antibody (Center) (Cat. #AP4959c) in MDA-MB435 cell line lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab.
  • WB - RRAGD Antibody  (Center) AP4959c
    Western blot analysis of RRAGD Antibody (Center) (Cat. #AP4959c) in mouse cerebellum tissue lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab.
  • IHC-P - RRAGD Antibody  (Center) AP4959c
    RRAGD Antibody (Center) (Cat. #AP4959c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RRAGD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - RRAGD Antibody  (Center) AP4959c
    RRAGD Antibody (Center) (Cat. #AP4959c) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NQL2
Other Accession Q7TT45, Q99K70, Q9HB90
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 45588 Da
Antigen Region 171-199 aa
Additional Information
Gene ID 58528
Other Names Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903)
Target/Specificity This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRRAGD Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RRAGD (HGNC:19903)
Function Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Cellular Location Cytoplasm. Nucleus. Lysosome. Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA
Research Areas
Citations (0)

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RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.


Olsen, J.V., et al. Cell 127(3):635-648(2006)
de Wit, N.J., et al. Br. J. Cancer 92(12):2249-2261(2005)
Sekiguchi, T., et al. J. Biol. Chem. 279(9):8343-8350(2004)

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$ 295.00
$ 99.00
Cat# AP4959c
(40 western blots)
Availability: In Stock
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