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FA20A Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FA20A Antibody (N-term) AP4969a
    Western blot analysis of FA20A Antibody (N-term) (Cat. #AP4969a) in MCF-7 cell line lysates (35ug/lane). FA20A (arrow) was detected using the purified Pab.
    detail
  • IHC-P - FA20A Antibody (N-term) AP4969a
    FA20A Antibody (N-term) (Cat. #AP4969a) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FA20A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - FA20A Antibody (N-term) AP4969a
    FA20A Antibody (N-term) (Cat. #AP4969a) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q96MK3
Other Accession NP_060035
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 61417 Da
Antigen Region 48-77 aa
Additional Information
Gene ID 54757
Other Names Protein FAM20A, FAM20A
Target/Specificity This FA20A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the N-terminal region of human FA20A.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFA20A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FAM20A (HGNC:23015)
Function Pseudokinase that acts as an allosteric activator of the Golgi serine/threonine protein kinase FAM20C and is involved in biomineralization of teeth. Forms a complex with FAM20C and increases the ability of FAM20C to phosphorylate the proteins that form the 'matrix' that guides the deposition of the enamel minerals.
Cellular Location Secreted {ECO:0000250|UniProtKB:Q8CID3}. Golgi apparatus. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q8CID3}
Tissue Location Highly expressed in lung and liver. Intermediate levels in thymus and ovary.
Research Areas
Citations (0)
citation

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References

Nalbant, D., et al. BMC Genomics 6 (1), 11 (2005)

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$ 365.00
$ 140.00
Cat# AP4969a
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