|Application ||WB, IHC-P, E|
|Other Accession||Q14532, NP_002271|
|Calculated MW||50361 Da|
|Antigen Region||352-380 aa|
|Other Names||Keratin, type I cuticular Ha5, Hair keratin, type I Ha5, Keratin-35, K35, KRT35, HHA5, HKA5, KRTHA5|
|Target/Specificity||This KRT35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-380 amino acids from the C-terminal region of human KRT35.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KRT35 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HHA5, HKA5, KRTHA5|
|Tissue Location||Early expression in the hair follicle, mainly found in supramatricial cells and lowermost cortical cells of the hair bulb.|
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Provided below are standard protocols that you may find useful for product applications.
KRT35 is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.
Gilon, M., et al. Differentiation 76(5):518-530(2008)
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
Cribier, B., et al. J. Invest. Dermatol. 122(5):1078-1083(2004)
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