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ARHGAP30 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARHGAP30 Antibody (C-term) AP4996b
    (LEFT)Western blot analysis of ARHGAP30 Antibody (C-term) (Cat. #AP4996b) in Ramos cell line lysates (35ug/lane).ARHGAP30 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of ARHGAP30 Antibody (C-term) (Cat. #AP4996b) in mouse NIH-3T3 cell line lysates (35ug/lane).ARHGAP30 (arrow) was detected using the purified Pab.
    detail
  • FC - ARHGAP30 Antibody (C-term) AP4996b
    ARHGAP30 Antibody (C-term) (Cat. #AP4996b) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q7Z6I6
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 118582 Da
Antigen Region 784-812 aa
Additional Information
Gene ID 257106
Other Names Rho GTPase-activating protein 30, Rho-type GTPase-activating protein 30, ARHGAP30
Target/Specificity This ARHGAP30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-812 amino acids from the C-terminal region of human ARHGAP30.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARHGAP30 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARHGAP30
Function GTPase-activating protein (GAP) for RAC1 and RHOA, but not for CDC42.
Cellular Location Cytoplasmic vesicle
Research Areas
Citations ( 0 )

Background

ARHGAP30 is GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

References

Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004)

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$ 365.00
$ 140.00
Cat# AP4996b
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