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TPPP3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TPPP3 Antibody (C-term) AP5004b
    All lanes : Anti-TPPP3 Antibody (C-term) at 1:2000 dilution Lane 1: WiDr whole cell lysate Lane 2: Mouse cerebellum lysate Lane 3: Mouse skeletal muscle lysate Lane 4: Rat skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - TPPP3 Antibody (C-term) AP5004b
    TPPP3 Antibody (C-term) (Cat. #AP5004b) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TPPP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - TPPP3 Antibody (C-term) AP5004b
    TPPP3 Antibody (C-term) (Cat. #AP5004b) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9BW30
Other Accession Q5PPN5, Q9CRB6, Q3ZCC8
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 18985 Da
Antigen Region 117-146 aa
Additional Information
Gene ID 51673
Other Names Tubulin polymerization-promoting protein family member 3, TPPP/p20, TPPP3
Target/Specificity This TPPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids of human TPPP3.
Dilution WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTPPP3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPPP3
Function Binds tubulin and has microtubule bundling activity. May play a role in cell proliferation and mitosis.
Cellular Location Cytoplasm, cytoskeleton
Research Areas
Citations ( 0 )

Background

TPPP3 binds tubulin and has microtubule bundling activity. TPPP3 may play a role in cell proliferation and mitosis.

References

Zhou, W., et al. Mol. Cell. Biochem. 333 (1-2), 91-98 (2010) Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009) Vincze, O., et al. Biochemistry 45(46):13818-13826(2006)

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$ 295.00
$ 99.00
Cat# AP5004b
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(40 western blots)
Availability: In Stock
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