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>   home   >   Products   >   Primary Antibodies   >   B3GALT6 Antibody (C-term)   

B3GALT6 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - B3GALT6 Antibody (C-term) AP5011b
    Western blot analysis of B3GALT6 Antibody (C-term) (Cat. #AP5011b) in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) was detected using the purified Pab.
  • IHC-P - B3GALT6 Antibody (C-term) AP5011b
    B3GALT6 Antibody (C-term) (Cat. #AP5011b) in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) ) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B3GALT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - B3GALT6 Antibody (C-term) AP5011b
    B3GALT6 Antibody (C-term) (Cat. #AP5011b) flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q96L58
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 37138 Da
Antigen Region 241-269 aa
Additional Information
Gene ID 126792
Other Names Beta-1, 3-galactosyltransferase 6, Beta-1, 3-GalTase 6, Beta3Gal-T6, Beta3GalT6, GAG GalTII, Galactosyltransferase II, Galactosylxylosylprotein 3-beta-galactosyltransferase, UDP-Gal:betaGal beta 1, 3-galactosyltransferase polypeptide 6, B3GALT6
Target/Specificity This B3GALT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-269 amino acids from the C-terminal region of human B3GALT6.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB3GALT6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B3GALT6
Function Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues.
Cellular Location Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein
Tissue Location Ubiquitous..
Citations (0)

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Background

B3GALT6, Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. B3GALT6 has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. B3GALT6 has no activity towards substrates with terminal glucosamine or galactosamine residues.

References

Bai, X., et al. J. Biol. Chem. 276(51):48189-48195(2001) Zhou, D., et al. Proc. Natl. Acad. Sci. U.S.A. 96(2):406-411(1999)

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Cat# AP5011b
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(40 western blots)
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