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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ID1 Antibody (Center)   

ID1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - ID1 Antibody (Center) AP50188
    Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (AP50188) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • IHC-P - ID1 Antibody (Center) AP50188
    Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (AP50188) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • IHC-P - ID1 Antibody (Center) AP50188
    Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (AP50188) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • WB - ID1 Antibody (Center) AP50188
    Western blot analysis of lysate from HepG2 cell line,using ID1 Antibody (Center)(AP50188). AP50188 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P41134
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=16;M=18,16;R=17,16; KDa
Antigen Region 51-155/155 human aa
Additional Information
Gene ID 3397
Other Names DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, bHLHb24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, ID1, BHLHB24, ID
Dilution IHC-P~~1:100~1:500
WB~~ 1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ID1
Synonyms BHLHB24, ID
Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).
Cellular Location Cytoplasm. Nucleus.
Research Areas
Citations (0)

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Background

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

References

Deed R.W.,et al.Biochim. Biophys. Acta 1219:160-162(1994).
Hara E.,et al.J. Biol. Chem. 269:2139-2145(1994).
Zhu W.,et al.Brain Res. Mol. Brain Res. 30:312-326(1995).
Nehlin J.O.,et al.Biochem. Biophys. Res. Commun. 231:628-634(1997).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
Cat# AP50188
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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