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PDLIM1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - PDLIM1 Antibody AP50197
    Immunofluorescence analysis of HepG2 cells, using PDLIM1 antibody.
    detail
  • WB - PDLIM1 Antibody AP50197
    Western blot analysis of lysates from Jurkat,HepG2 cell line (from left to right),using PDLIM1 Antibody(C10012). C10012 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
    detail
  • IHC - PDLIM1 Antibody AP50197
    Immunohistochemistry analysis of paraffin-embedded human heart tissue using PDLIM1 antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession O00151
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 36 KDa
Antigen Region 91-119 aa
Additional Information
Gene ID 9124
Other Names PDZ and LIM domain protein 1, C-terminal LIM domain protein 1, Elfin, LIM domain protein CLP-36, PDLIM1, CLIM1, CLP36
Dilution IF~~1:100
WB~~ 1:1000
IHC~~1:50-1:100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name PDLIM1
Synonyms CLIM1, CLP36
Function Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line Note=Associates with actin stress fibers
Tissue Location Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes
Research Areas
Citations (0)
citation

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Background

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.

References

Kotaka M.,et al.J. Cell. Biochem. 72:279-285(1999).
Bauer K.,et al.Blood 96:4236-4245(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).

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$ 350.00
Cat# AP50197
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