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FOLR2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FOLR2 Antibody (N-term) AP5032a
    Western blot analysis of lysate from human placenta tissue, using FOLR2 Antibody (N-term)(Cat. #AP5032a). AP5032a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - FOLR2 Antibody (N-term) AP5032a
    Western blot analysis of lysates from MDA-MB-453 cell line, human placenta tissue lysate (from left to right), using FOLR2 Antibody (N-term) (Cat. #AP5032a). AP5032a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - FOLR2 Antibody (N-term) AP5032a
    Western blot analysis of lysate from human placenta tissue lysate, using FOLR2 Antibody (N-term)(Cat. #AP5032a). AP5032a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - FOLR2 Antibody (N-term) AP5032a
    Western blot analysis of lysate from human placenta tissue lysate, using FOLR2 Antibody (N-term)(Cat. #AP5032a). AP5032a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - FOLR2 Antibody (N-term) AP5032a
    Western blot analysis of lysates from KG-1, MCF-7, MDA-MB-453 cell line (from left to right), using FOLR2 Antibody (N-term)(Cat. #AP5032a). AP5032a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - FOLR2 Antibody (N-term) AP5032a
    Western blot analysis of lysate from mouse heart tissue lysate, using FOLR2 Antibody (N-term)(Cat. #AP5032a). AP5032a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • IHC-P - FOLR2 Antibody (N-term) AP5032a
    FOLR2 Antibody (N-term) (Cat. #AP5032a) IHC analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FOLR2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - FOLR2 Antibody (N-term) AP5032a
    FOLR2 Antibody (N-term) (Cat. #AP5032a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P14207
Other Accession Q05685
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29280 Da
Antigen Region 18-46 aa
Additional Information
Gene ID 2350
Other Names Folate receptor beta, FR-beta, Folate receptor 2, Folate receptor, fetal/placental, Placental folate-binding protein, FBP, FOLR2
Target/Specificity This FOLR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids of human FOLR2.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOLR2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOLR2
Function Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor. Secreted
Tissue Location Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues
Research Areas
Citations ( 0 )

Background

FOLR2 is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.

References

Puig-Kroger, A., et al. Cancer Res. 69(24):9395-9403(2009) Boyles, A.L., et al. Genet. Epidemiol. 33(3):247-255(2009) Franke, B., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 85(3):216-226(2009)

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$ 295.00
$ 99.00
Cat# AP5032a
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Quantity:
(40 western blots)
Availability: In Stock
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