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HSPA5 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HSPA5 Antibody (C-term) AP5041b
    HSPA5 Antibody (C-term) (Cat.#AP5041b) western blot analysis in A375,A2058,Hela cell line lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).
  • IHC-P - HSPA5 Antibody (C-term) AP5041b
    HSPA5 Antibody (C-term) (Cat. #AP5041b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSPA5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - HSPA5 Antibody (C-term) AP5041b
    HSPA5 Antibody (C-term) (Cat. #AP5041b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11021
Other Accession Q91883, P06761, P34935, P20029, Q0VCX2, NP_005338
Reactivity Human
Predicted Bovine, Mouse, Pig, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 72333 Da
Antigen Region 541-568 aa
Additional Information
Gene ID 3309
Other Names 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78
Target/Specificity This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-568 amino acids from the C-terminal region of human HSPA5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPA5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPA5
Synonyms GRP78
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Citations (0)

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When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.


Zhao, C., et al. J. Med. Virol. 82(1):14-22(2010)
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Arnaudeau, S., et al. Proteomics 9(23):5316-5327(2009)

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$ 295.00
$ 99.00
Cat# AP5041b
(40 western blots)
Availability: In Stock
Bulk Size
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