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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   LIMD2 Antibody (N-term)   

LIMD2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LIMD2 Antibody (N-term) AP5051a
    Western blot analysis of LIMD2 Antibody (N-term) (Cat. #AP5051a) in Jurkat cell line lysates (35ug/lane).LIMD2 (arrow) was detected using the purified Pab.
  • IHC-P - LIMD2 Antibody (N-term) AP5051a
    LIMD2 Antibody (N-term) (Cat. #AP5051a) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LIMD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - LIMD2 Antibody (N-term) AP5051a
    LIMD2 Antibody (N-term) (Cat. #AP5051a) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9BT23
Other Accession Q4KM31, Q8BGB5, Q1LZA7
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14070 Da
Antigen Region 84-113 aa
Additional Information
Gene ID 80774
Other Names LIM domain-containing protein 2, LIMD2
Target/Specificity This LIMD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-113 amino acids from the N-terminal region of human LIMD2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLIMD2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIMD2 (HGNC:28142)
Function Acts as an activator of the protein-kinase ILK, thereby regulating cell motility (PubMed:24590809).
Cellular Location Cytoplasm. Nucleus. Note=Mainly found in cytoplasm, concentrated in membrane ruffles and in streaks reminiscent of focal adhesion plaques (PubMed:24590809). Also found in nucleus (PubMed:24590809).
Research Areas
Citations (0)

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References

Fu, G.K., et al. Genomics 84(1):205-210(2004)

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Cat# AP5051a
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(40 western blots)
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