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NXF1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NXF1 Antibody AP50580
    Western blot analysis of lysates from Hela,293T cell line (from left to right),using NXF1 Antibody(AP50580). AP50580 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9UBU9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 70,40 KDa
Antigen Region 5-33 aa
Additional Information
Gene ID 10482
Other Names Nuclear RNA export factor 1, Tip-associated protein, Tip-associating protein, mRNA export factor TAP, NXF1, TAP
Dilution WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name NXF1
Synonyms TAP
Function Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export.
Cellular Location Nucleus, nucleoplasm Nucleus speckle. Cytoplasm. Nucleus. Note=Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. The association with the TREX complex seems to occur in regions surrounding nuclear speckles known as perispeckles (PubMed:23826332). Nucleus; nuclear rim (PubMed:25662211).
Tissue Location Expressed ubiquitously.
Citations (0)

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Background

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export.

References

Braun I.C.,et al.EMBO J. 18:1953-1965(1999).
Kang Y.,et al.Genes Dev. 13:1126-1139(1999).
Bear J.,et al.Mol. Cell. Biol. 19:6306-6317(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

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$ 295.00
Cat# AP50580
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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