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CREBZF Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - CREBZF Antibody AP50597
    Immunofluorescence analysis of HepG2 cells, using CREBZF antibody.
    detail
  • WB - CREBZF Antibody AP50597
    Western blot analysis of lysates from Hela,PC-3 cell line (from left to right),using CREBZF Antibody(AP50597). AP50597 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q9NS37
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 37 KDa
Antigen Region 232-264 aa
Additional Information
Gene ID 58487
Other Names CREB/ATF bZIP transcription factor, Host cell factor-binding transcription factor Zhangfei, HCF-binding transcription factor Zhangfei, CREBZF, ZF
Dilution IF~~1:100
WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name CREBZF
Synonyms ZF
Function Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells.
Cellular Location Nucleus. Note=Colocalizes in promyelocytic leukemia protein nuclear bodies (PML- NB) with CREB3 and HCFC1
Tissue Location In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver
Citations (0)
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Background

Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1- dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells.

References

Lu R.,et al.Nucleic Acids Res. 28:2446-2454(2000).
Taylor T.D.,et al.Nature 440:497-500(2006).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zhang J.W.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Misra V.,et al.J. Biol. Chem. 280:15257-15266(2005).

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$ 350.00
Cat# AP50597
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