|Application ||WB, FC, E|
|Other Accession||Q6GP53, Q6PGB6, Q6DBY2, Q0IIJ0|
|Predicted||Bovine, Zebrafish, Mouse, Xenopus|
|Calculated MW||19398 Da|
|Antigen Region||117-146 aa|
|Other Names||N-alpha-acetyltransferase 50, 231-, N-acetyltransferase 13, N-acetyltransferase 5, hNAT5, N-acetyltransferase san homolog, hSAN, NatE catalytic subunit, NAA50, MAK3, NAT13, NAT5|
|Target/Specificity||This NAT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human NAT13.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NAT13 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine (PubMed:19744929, PubMed:22311970, PubMed:21900231, PubMed:27484799). Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position (PubMed:27484799). Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins (PubMed:19744929). Autoacetylates in vivo (PubMed:19744929). The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo (PubMed:19744929). Component of a N-alpha- acetyltransferase complex containing NAA10 and NAA15, but NAA50 does not influence the acetyltransferase activity of NAA10: this multiprotein complex probably constitutes the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides (PubMed:16507339, PubMed:27484799). Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10 (PubMed:17502424, PubMed:27422821).|
|Cellular Location||Cytoplasm. Nucleus. Note=Localizes to the cytoplasm in interphase cells (PubMed:17502424).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
NAT13 is probable catalytic component of the ARD1A-NARG1 complex which displays alpha (N-terminal) acetyltransferase activity.
Starheim, K.K., et al. Mol. Cell. Biol. 29(13):3569-3581(2009)
Polevoda, B., et al. BMC Proc 3 SUPPL 6, S2 (2009)
Hou, F., et al. J. Cell Biol. 177(4):587-597(2007)
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