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PAAF1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PAAF1 Antibody (N-term) AP5062a
    Western blot analysis of PAAF1 Antibody (N-term) (Cat. #AP5062a) in A549 cell line lysates (35ug/lane).PAAF1 (arrow) was detected using the purified Pab.
  • IHC-P - PAAF1 Antibody (N-term) AP5062a
    PAAF1 Antibody (N-term) (Cat. #AP5062a) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAAF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9BRP4
Other Accession Q148I1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42190 Da
Antigen Region 109-138 aa
Additional Information
Gene ID 80227
Other Names Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71, PAAF1, WDR71
Target/Specificity This PAAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the N-terminal region of human PAAF1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAAF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAAF1
Synonyms WDR71
Function Inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency. Protects SUPT6H from proteasomal degradation.
Tissue Location Ubiquitously expressed, with highest levels in kidney, brain and testis.
Citations (0)

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Background

PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency.

References

Lassot, I., et al. Mol. Cell 25(3):369-383(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Ponnappan, S., et al. Int. J. Biochem. Cell Biol. 39(4):799-809(2007)

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$ 295.00
$ 99.00
Cat# AP5062a
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Quantity:
(40 western blots)
Availability: In Stock
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