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NFYA Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NFYA Antibody AP50674
    Western blot analysis of lysate from Jurkat cell line,using NFYA Antibody(AP50674). AP50674 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23511
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 37 34 KDa
Antigen Region 280-309 aa
Additional Information
Gene ID 4800
Other Names Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA, NFYA
Dilution WB~~1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'- CCAAT-3' box motif found in the promoters of its target genes. NF- Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.
Cellular Location Nucleus.
Citations (0)

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Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.


Li X.-Y.,et al.Nucleic Acids Res. 20:1087-1091(1992).
Mungall A.J.,et al.Nature 425:805-811(2003).
Becker D.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:1968-1972(1991).
Yamada K.,et al.Biochem. Biophys. Res. Commun. 261:614-621(1999).
Yamada K.,et al.FEBS Lett. 460:41-45(1999).

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$ 295.00
Cat# AP50674
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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