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p47 phox (Ab-345) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - p47 phox (Ab-345) Antibody AP50679
    Western blot analysis of lysate from Daudi cell line,using p47 phox (Ab-345) Antibody(AP50679). AP50679 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P14598
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 45 31 KDa
Antigen Region 330-356 aa
Additional Information
Gene ID 653361
Other Names Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A
Dilution WB~~1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name NCF1
Synonyms NOXO2, SH3PXD1A
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Detected in peripheral blood monocytes and neutrophils (at protein level).
Research Areas
Citations (0)
citation

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Background

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

References

Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989).
Lomax K.J.,et al.Science 245:409-412(1989).
Rodaway A.R.F.,et al.Mol. Cell. Biol. 10:5388-5396(1990).
Gorlach A.,et al.J. Clin. Invest. 100:1907-1918(1997).

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$ 350.00
Cat# AP50679
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Availability: 2 weeks
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