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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Tubulin Beta Antibody   

Tubulin Beta Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Tubulin Beta Antibody AP50855
    Lane 1:Mouse heart lysates; Lane 2: Human MCF7 cell lysates probed with Rabbit Anti-Tubulin Beta Polyclonal Antibody, Unconjugated (AP50855) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
  • IHC-P - Tubulin Beta Antibody AP50855
    Formalin-fixed and paraffin embedded rat brain labeled with Anti-tubulin Beta Polyclonal Antibody, Unconjugated (AP50855) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • IF - Tubulin Beta Antibody AP50855
    Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37°C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(AP50855-Cy3) 1:200, 40 minutes at 37°C
  • WB - Tubulin Beta Antibody AP50855
    Lane 1:Mouse heart lysates; Lane 2: Human MCF7 cell lysates probed with Rabbit Anti-Tubulin Beta Polyclonal Antibody, Unconjugated AP50855 at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
  • IHC - Tubulin Beta Antibody AP50855
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-tubulin Beta Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P07437
Reactivity Human, Mouse, Rat, Rabbit
Host Rabbit
Clonality Polyclonal
Calculated MW 50 KDa
Antigen Region 61-160/444 aa
Additional Information
Gene ID 203068
Other Names Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
IF~~1:100~1:200
IHC~~1:100-1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name TUBB
Synonyms TUBB5
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Research Areas
Citations (0)

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Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

References

Lee M.G.-S.,et al.Cell 33:477-487(1983).
Hall J.L.,et al.Mol. Cell. Biol. 3:854-862(1983).
Crabtree D.V.,et al.Bioorg. Med. Chem. 9:1967-1976(2001).
Yu W.,et al.Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Shiina S.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
Cat# AP50855
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
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