|Application ||WB, IHC-P, E|
|Calculated MW||49754 Da|
|Antigen Region||281-309 aa|
|Other Names||UBX domain-containing protein 6, UBX domain-containing protein 1, UBXN6, UBXD1, UBXDC2|
|Target/Specificity||This UBXN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-309 amino acids from the C-terminal region of human UBXN6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBXN6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May negatively regulate the ATPase activity of VCP, an ATP-driven segregase that associates with different cofactors to control a wide variety of cellular processes (PubMed:26475856). As a cofactor of VCP, it may play a role in the transport of CAV1 to lysosomes for degradation (PubMed:21822278, PubMed:23335559). It may also play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins (PubMed:19275885). Together with VCP and other cofactors, it may play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes (PubMed:27753622).|
|Cellular Location||Cytoplasm. Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Early endosome membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein Note=Localizes at the centrosome both in interphase and during mitosis (PubMed:18656546). May be recruited to endosomal and lysosomal membranes as part of a ternary complex with CAV1 and VCP (PubMed:21822278). Recruited to damaged lysosomes decorated with K48-linked ubiquitin chains (PubMed:27753622)|
|Tissue Location||Enhanced expression in testis.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
UBXN6 acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. UBXN6 is MDM2 stabilization leads to MDM2-dependent TP53 degradation.
Nagahama, M., et al. Biochem. Biophys. Res. Commun. 382(2):303-308(2009)
Kern, M., et al. Biochem. Biophys. Res. Commun. 380(2):303-307(2009)
Madsen, L., et al. Int. J. Biochem. Cell Biol. 40(12):2927-2942(2008)
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