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XRCC6 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP5089b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP5089b-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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XRCC6 Antibody (C-term) - Product info | |
| Application | WB, IHC, FC, IF
|
| Primary Accession | P12956 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 69843 Da |
XRCC6 Antibody (C-term) - Additional info | |
| Gene ID 2547 | |
| Other Names XRCC6; G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Thyroid-lupus autoantigen; X-ray repair complementing defective repair in Chinese hamster cells 6 | |
| Target/Specificity This XRCC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-560 amino acids from the C-terminal region of human XRCC6. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions XRCC6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
XRCC6 Antibody (C-term) - Protein Information | |
| Name XRCC6 | |
| Synonyms G22P1 | |
| Function Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose- 5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription | |
| Cellular Location Nucleus. Chromosome. | |
XRCC6 Antibody (C-term) - Related products
AP5089b: XRCC6 Antibody (C-term)
AP5089c: XRCC6 Antibody (Center)
AP6775a: Ku70 Antibody (N-term)
AP6775c: Ku70 Antibody (Center)
RI15763: XRCC6 predesign siRNA
DC02375: Human XRCC6 cDNA Clone
LY10971a: XRCC6 Over-expression Lysate
BP5089b: XRCC6 Antibody (C-term) Blocking Peptide
BP5089c: XRCC6 Antibody (Center) Blocking peptide
BP6775a: Ku70 Antibody (N-term) Blocking Peptide
BP6775c: Ku70 Antibody (Center) Blocking Peptide
AT2136a: G22P1 Antibody (monoclonal) (M01)
XRCC6 Antibody (C-term) - Application data
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Western blot analysis of XRCC6 Antibody (C-term) (Cat. #AP5089b) in A2058 cell line lysates (35ug/lane).XRCC6 (arrow) was detected using the purified Pab.
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XRCC6 Antibody (C-term) (Cat. #AP5089b) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Western blot analysis of XRCC6 (arrow) using rabbit polyclonal XRCC6 Antibody (C-term) (Cat. #AP5089b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XRCC6 gene.
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XRCC6 Antibody (C-term) (Cat. #AP5089b) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of XRCC6 Antibody (C-term)(Cat#AP5089b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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Fluorescent confocal image of Hela cell stained with XRCC6 Antibody (C-term)(Cat#AP5089b). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with XRCC6 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). XRCC6 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
XRCC6 Antibody (C-term) - Research Areas
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BACKGROUND
XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
REFERENCES
Vishnudas, V.K., et al. Hum. Mol. Genet. 18(23):4467-4477(2009) Wang, Q., et al. Neoplasia 11(10):1012-1021(2009) Beskow, C., et al. Br. J. Cancer 101(5):816-821(2009)
