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GLD2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - GLD2 Antibody (N-term) AP5092a
    Fluorescent image of MCF-7 cells stained with GLD2 Antibody (N-term)(Cat#AP5092a). AP5092a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • IHC-P - GLD2 Antibody (N-term) AP5092a
    Immunohistochemical analysis of paraffin-embedded H. cerebellum section using GLD2 Antibody (N-term)(Cat#AP5092a). AP5092a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - GLD2 Antibody (N-term) AP5092a
    Western blot analysis of lysates from mouse brain, mouse cerebellum tissue lysate (from left to right), using GLD2 Antibody (N-term)(Cat. #AP5092a). AP5092a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - GLD2 Antibody (N-term) AP5092a
    Western blot analysis of lysates from HepG2 cell line and mouse brain tissue lysate (from left to right), using GLD2 Antibody (N-term)(Cat. #AP5092a). AP5092a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • IHC-P - GLD2 Antibody (N-term) AP5092a
    GLD2 Antibody (N-term) (Cat. #AP5092a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q6PIY7
Other Accession Q2HJ44
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56028 Da
Antigen Region 58-87 aa
Additional Information
Gene ID 167153
Other Names Poly(A) RNA polymerase GLD2, hGLD-2, PAP-associated domain-containing protein 4, Terminal uridylyltransferase 2, TUTase 2, PAPD4, GLD2
Target/Specificity This GLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human GLD2.
Dilution IF~~1:25
IHC-P~~1:50~100
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLD2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAPD4
Synonyms GLD2
Function Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation.
Cellular Location Cytoplasm. Nucleus
Tissue Location Expressed in brain. Within brain, it is expressed in cerebellum, hippocampus and medulla
Research Areas
Citations ( 0 )

Background

GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.

References

Mullen, T.E., et al. Genes Dev. 22(1):50-65(2008) Kwak, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 101(13):4407-4412(2004)

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$ 295.00
$ 99.00
Cat# AP5092a
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(40 western blots)
Availability: In Stock
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