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ADIPOR2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ADIPOR2 Antibody (Center) AP5105C
    Confocal immunofluorescent analysis of ADIPOR2 Antibody (Center) (Cat. #AP5105c) with Hela cell followed by Alexa Fluor搴?489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - ADIPOR2 Antibody (Center) AP5105C
    Western blot analysis of ADIPOR2 Antibody (Center) (Cat. #AP5105c) in Hela cell line lysates (35ug/lane).ADIPOR2 (arrow) was detected using the purified Pab.
    detail
  • WB - ADIPOR2 Antibody (Center) AP5105C
    All lanes : Anti-ADIPOR2 Antibody (Center) at 1:2000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ADIPOR2 Antibody (Center) AP5105C
    Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ADIPOR2 Antibody (Center) AP5105C
    Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human skeletal muscle tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - ADIPOR2 Antibody (Center) AP5105C
    Overlay histogram showing U-2 OS cells stained with AP5105c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP5105c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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  • SPECIFICATION
  • CITATIONS: 2
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, FC, E
Primary Accession Q86V24
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43884 Da
Antigen Region 45-72 aa
Additional Information
Gene ID 79602
Other Names Adiponectin receptor protein 2, Progestin and adipoQ receptor family member II, ADIPOR2, PAQR2
Target/Specificity This ADIPOR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-72 amino acids from the Central region of human ADIPOR2.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADIPOR2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADIPOR2 (HGNC:24041)
Function Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed:12802337, PubMed:25855295). Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein Note=Localized to the cell membrane and intracellular organelles
Tissue Location Ubiquitous (PubMed:16044242). Highly expressed in skeletal muscle, liver and placenta (PubMed:12802337). Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung (PubMed:12802337)
Research Areas
Citations ( 0 )

Background

The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin.

References

Ferguson, J.F., et al. Am. J. Clin. Nutr. 91(3):794-801(2010) Halvatsiotis, I., et al. Cardiovasc Diabetol 9, 10 (2010) Yoshihara, K., et al. J Reprod Med 54 (11-12), 669-674 (2009)

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$ 182.50
$ 70.00
Cat# AP5105C
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