|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8AVM5, P25286, Q9Z1G4, Q9I8D0, Q29466|
|Predicted||Bovine, Chicken, Mouse, Rat, Xenopus|
|Calculated MW||96413 Da|
|Antigen Region||44-71 aa|
|Other Names||V-type proton ATPase 116 kDa subunit a isoform 1, V-ATPase 116 kDa isoform a1, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1, ATP6V0A1, ATP6N1, ATP6N1A, VPP1|
|Target/Specificity||This ATP6V0A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the N-terminal region of human ATP6V0A1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP6V0A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ATP6N1, ATP6N1A, VPP1|
|Function||Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).|
|Cellular Location||Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note=Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
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Provided below are standard protocols that you may find useful for product applications.
ATP6V0A1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles.
Antonacopoulou, A.G., et al. Anticancer Res. 28 (2B), 1221-1227 (2008)
Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
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