|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8AVM5, P25286, Q9Z1G4, Q9I8D0, Q29466|
|Predicted||Bovine, Chicken, Mouse, Rat, Xenopus|
|Calculated MW||96413 Da|
|Antigen Region||44-71 aa|
|Other Names||V-type proton ATPase 116 kDa subunit a isoform 1, V-ATPase 116 kDa isoform a1, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1, ATP6V0A1, ATP6N1, ATP6N1A, VPP1|
|Target/Specificity||This ATP6V0A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the N-terminal region of human ATP6V0A1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP6V0A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ATP6N1, ATP6N1A, VPP1|
|Function||Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).|
|Cellular Location||Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note=Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
ATP6V0A1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles.
Antonacopoulou, A.G., et al. Anticancer Res. 28 (2B), 1221-1227 (2008)
Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.