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ATP6V0A1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ATP6V0A1 Antibody (N-term) AP5109A
    Western blot analysis of ATP6V0A1 Antibody (N-term) (Cat. #AP5109a) in K562 cell line lysates (35ug/lane).ATP6V0A1 (arrow) was detected using the purified Pab.
  • IHC-P - ATP6V0A1 Antibody (N-term) AP5109A
    ATP6V0A1 Antibody (N-term) (Cat. #AP5109A)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP6V0A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ATP6V0A1 Antibody (N-term) AP5109A
    ATP6V0A1 Antibody (N-term) (Cat. #AP5109a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q93050
Other Accession Q8AVM5, P25286, Q9Z1G4, Q9I8D0, Q29466
Reactivity Human
Predicted Bovine, Chicken, Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 96413 Da
Antigen Region 44-71 aa
Additional Information
Gene ID 535
Other Names V-type proton ATPase 116 kDa subunit a isoform 1, V-ATPase 116 kDa isoform a1, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1, ATP6V0A1, ATP6N1, ATP6N1A, VPP1
Target/Specificity This ATP6V0A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the N-terminal region of human ATP6V0A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP6V0A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP6V0A1
Synonyms ATP6N1, ATP6N1A, VPP1
Function Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).
Cellular Location Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note=Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Citations (0)

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ATP6V0A1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles.


Antonacopoulou, A.G., et al. Anticancer Res. 28 (2B), 1221-1227 (2008)
Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)

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Cat# AP5109A
(40 western blots)

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