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ZFYVE16 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ZFYVE16 Antibody (N-term) AP5120A
    Western blot analysis of ZFYVE16 Antibody (N-term) (Cat. #AP5120a) in K562 cell line lysates (35ug/lane).ZFYVE16 (arrow) was detected using the purified Pab.
  • IHC-P - ZFYVE16 Antibody (N-term) AP5120A
    ZFYVE16 Antibody (N-term) (Cat. #AP5120a) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZFYVE16 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ZFYVE16 Antibody (N-term) AP5120A
    ZFYVE16 Antibody (N-term) (Cat. #AP5120a) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z3T8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 168903 Da
Antigen Region 375-404 aa
Additional Information
Gene ID 9765
Other Names Zinc finger FYVE domain-containing protein 16, Endofin, Endosome-associated FYVE domain protein, ZFYVE16, KIAA0305
Target/Specificity This ZFYVE16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-404 amino acids from the N-terminal region of human ZFYVE16.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZFYVE16 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZFYVE16
Synonyms KIAA0305
Function May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
Cellular Location Cytoplasm. Early endosome membrane; Peripheral membrane protein. Note=Localized to early endosomes. Membrane-associated, probably via its association with phosphatidylinositol 3-phosphate (PI3P)
Tissue Location Widely expressed. Highly expressed in kidney, placenta and lung. Expressed at intermediate level in heart, brain, skeletal muscle, spleen and liver. Weakly expressed in colon, thymus and peripheral blood lymphocytes
Research Areas
Citations (0)

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The ZFYVE16 gene encodes endofin, an endosomal protein implicated in regulating membrane trafficking. It is characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain positioned in the middle of the molecule.


Toy, W., et al. Cell. Signal. 22(3):437-446(2010) Chen, Y., et al. Proteomics 7(14):2384-2397(2007) Shi, W., et al. J. Cell. Sci. 120 (PT 7), 1216-1224 (2007)

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Cat# AP5120A
(40 western blots)
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