|Application ||WB, IHC-P, FC, E|
|Antigen Region||72-100 aa|
|Other Names||28S ribosomal protein S33, mitochondrial, MRP-S33, S33mt, MRPS33|
|Target/Specificity||This RT33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-100 amino acids from the C-terminal region of human RT33.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RT33 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Mitochondrion. EMBL; AF151897; AAD34134.1; -; mRNA EMBL; AF078858; AAD44490.1; -; mRNA EMBL; AC006006; AAD43190.1; -; Genomic_DNA EMBL; BC015462; AAH15462.1; -; mRNA CCDS; CCDS5864.1; - RefSeq; NP_057155.1; NM_016071.3 RefSeq; NP_444263.1; NM_053035.2 UniGene; Hs.416207; - PDB; 3J9M; EM; 3.50 A; AZ=1-106 PDBsum; 3J9M; - ProteinModelPortal; Q9Y291; - SMR; Q9Y291; - BioGrid; 119658; 26 IntAct; Q9Y291; 5 STRING; 9606.ENSP00000320567; - iPTMnet; Q9Y291; - PhosphoSitePlus; Q9Y291; - BioMuta; MRPS33; - DMDM; 24212388; - EPD; Q9Y291; - MaxQB; Q9Y291; - PaxDb; Q9Y291; - PeptideAtlas; Q9Y291; - PRIDE; Q9Y291; - TopDownProteomics; Q9Y291; - Ensembl; ENST00000324787; ENSP00000320567; ENSG00000090263 Ensembl; ENST00000393008; ENSP00000376732; ENSG00000090263 Ensembl; ENST00000469351; ENSP00000417287; ENSG00000090263 Ensembl; ENST00000496958; ENSP00000418451; ENSG00000090263 GeneID; 51650; - KEGG; hsa:51650; - UCSC; uc003vwd.5; human CTD; 51650; - DisGeNET; 51650; - GeneCards; MRPS33; - HGNC; HGNC:16634; MRPS33 HPA; HPA030425; - MIM; 611993; gene neXtProt; NX_Q9Y291; - OpenTargets; ENSG00000090263; - PharmGKB; PA31020; - eggNOG; KOG4104; Eukaryota eggNOG; ENOG4111X83; LUCA GeneTree; ENSGT00390000011401; - HOVERGEN; HBG036129; - InParanoid; Q9Y291; - KO; K17411; - OMA; KEVYDWY; - OrthoDB; EOG091G0Y05; - PhylomeDB; Q9Y291; - TreeFam; TF313928; - BioCyc; ZFISH:ENSG00000090263-MONOMER; - Reactome; R-HSA-5368286; Mitochondrial translation initiation Reactome; R-HSA-5389840; Mitochondrial translation elongation Reactome; R-HSA-5419276; Mitochondrial translation termination ChiTaRS; MRPS33; human GeneWiki; MRPS33; - GenomeRNAi; 51650; - PRO; PR:Q9Y291; - Proteomes; UP000005640; Chromosome 7 Bgee; ENSG00000090263; - CleanEx; HS_MRPS33; - ExpressionAtlas; Q9Y291; baseline and differential Genevisible; Q9Y291; HS GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome GO; GO:0005763; C:mitochondrial small ribosomal subunit; NAS:UniProtKB GO; GO:0005739; C:mitochondrion; IBA:GO_Central GO; GO:0003735; F:structural constituent of ribosome; NAS:UniProtKB GO; GO:0070125; P:mitochondrial translational elongation; TAS:Reactome GO; GO:0070126; P:mitochondrial translational termination; TAS:Reactome GO; GO:0006412; P:translation; NAS:UniProtKB InterPro; IPR013219; Ribosomal_S27/S33_mit Pfam; PF08293; MRP-S33; 1 1: Evidence at protein level; 3D-structure; Acetylation; Complete proteome; Mitochondrion; Reference proteome; Ribonucleoprotein; Ribosomal protein INIT_MET 1 1 Removed. CHAIN 2 106 28S ribosomal protein S33, mitochondrial /FTId=PRO_0000087727 MOD_RES 2 2 N-acetylserine SEQUENCE 106 AA; 12629 MW; E755EDAFB6AF6A46 CRC64; MSSLSEYAFR MSRLSARLFG EVTRPTNSKS MKVVKLFSEL PLAKKKETYD WYPNHHTYAE LMQTLRFLGL YRDEHQDFMD EQKRLKKLRG KEKPKKGEGK RAAKRK|
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Provided below are standard protocols that you may find useful for product applications.
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans.
Tsuritani, K., et al. Genome Res. 17(7):1005-1014(2007) Zhang, Z., et al. Genomics 81(5):468-480(2003) Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)
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