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IFT20 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - IFT20 Antibody (Center) AP5133C
    Western blot analysis of IFT20 Antibody (Center) (Cat. #AP5133c) in mouse lung tissue lysates (35ug/lane).IFT20 (arrow) was detected using the purified Pab.
  • IHC-P - IFT20 Antibody (Center) AP5133C
    IFT20 Antibody (Center) (Cat. #AP5133c) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IFT20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8IY31
Other Accession Q61025, Q58CS6
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 15281 Da
Antigen Region 44-71 aa
Additional Information
Gene ID 90410
Other Names Intraflagellar transport protein 20 homolog, hIFT20, IFT20
Target/Specificity This IFT20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the Central region of human IFT20.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIFT20 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFT20
Function Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
Cellular Location Golgi apparatus, cis-Golgi network. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body. Note=Present at the centrosomes during the cell cycle and associated with the proximal portion of the mother centriole and the lateral aspect of the daughter centriole. Associated with basal body at the base of primary cilia (By similarity).
Tissue Location Expressed in almost all tissues.
Research Areas
Citations (0)

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IFT20 is part of intraflagellar transport (IFT) particles involved in ciliary process assembly. IFT20 may play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.


Follit, J.A., et al. Mol. Biol. Cell 17(9):3781-3792(2006)
Jurczyk, A., et al. J. Cell Biol. 166(5):637-643(2004)
Yin, G., et al. Mol. Biol. Rep. 30(4):255-260(2003)

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Cat# AP5133C
(40 western blots)
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