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SAP14 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SAP14 Antibody AP51508
    Anti-SAP14 Antibodyat 1:1000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9Y3B4
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 14 KDa
Additional Information
Gene ID 51639
Other Names Splicing factor 3B subunit 6, Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, SF3B14a, Spliceosome-associated protein, 14-kDa, Splicing factor 3b, subunit 6, 14kDa, SF3B6, SAP14, SF3B14, SF3B14A
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name SF3B6
Synonyms SAP14, SF3B14, SF3B14A
Function Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
Cellular Location Nucleus.
Citations (0)

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Background

Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.

References

Will C.L.,et al.EMBO J. 20:4536-4546(2001).
Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Will C.L.,et al.EMBO J. 21:4978-4988(2002).

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$ 295.00
Cat# AP51508
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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