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POLR2I Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - POLR2I Antibody AP51763
    All lanes : Anti-POLR2I Antibody at 1:4000 dilution Lane 1: A549 whole cell lysates Lane 2: A431 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P36954
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 14 KDa
Additional Information
Gene ID 5438
Other Names DNA-directed RNA polymerase II subunit RPB9, RNA polymerase II subunit B9, DNA-directed RNA polymerase II subunit I, RNA polymerase II 145 kDa subunit, RPB145, POLR2I
Dilution WB~~ 1:4000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name POLR2I
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity).
Cellular Location Nucleus, nucleolus
Citations (0)

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Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity).

References

Acker J.,et al.Nucleic Acids Res. 21:5345-5350(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Kershnar E.,et al.J. Biol. Chem. 273:34444-34453(1998).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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$ 295.00
Cat# AP51763
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
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