POLR2I Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P36954 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 14 KDa |
Antigen Region | 31 - 90 aa |
Gene ID | 5438 |
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Other Names | DNA-directed RNA polymerase II subunit RPB9, RNA polymerase II subunit B9, DNA-directed RNA polymerase II subunit I, RNA polymerase II 145 kDa subunit, RPB145, POLR2I |
Target/Specificity | KLH conjugated synthetic peptide derived from human POLR2I |
Dilution | WB~~ 1:4000 |
Format | 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50% |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | POLR2I |
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Function | Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2I/RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template. |
Cellular Location | Nucleus, nucleolus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity).
References
Acker J.,et al.Nucleic Acids Res. 21:5345-5350(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Kershnar E.,et al.J. Biol. Chem. 273:34444-34453(1998).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
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