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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-Smad7 + Smad6 Polyclonal Antibody   

Rabbit Anti-Smad7 + Smad6 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Rabbit Anti-Smad7 + Smad6 Polyclonal Antibody AP52036
    Mouse placenta lysate probed with Anti-Smad7 + Smad6 Polyclonal Antibody (AP52036) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody at 1:5000 90min in 37˚C.
    detail
  • WB - Rabbit Anti-Smad7 + Smad6 Polyclonal Antibody AP52036
    Mouse lung lysate probed with Anti-Smad7 + Smad6 Polyclonal Antibody (AP52036 at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody antibody at 1:5000 90min in 37˚C.
    detail
  • IHC-P - Rabbit Anti-Smad7 + Smad6 Polyclonal Antibody AP52036
    Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Smad7/Smad6 Polyclonal Antibody (AP52036), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession O15105
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 46426 Da
Additional Information
Gene ID 4092
Other Names CRCS3; MADH7; MADH8; Mothers against decapentaplegic homolog 7; MAD homolog 7; Mothers against DPP homolog 7; Mothers against decapentaplegic homolog 8; MAD homolog 8; Mothers against DPP homolog 8; SMAD family member 7; SMAD 7; Smad7; hSMAD7
Dilution WB~~1:100-1:500
IHC-P~~1:100~1:500
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name SMAD7
Synonyms MADH7, MADH8
Function Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access (PubMed:21791611). Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Location Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236)
Tissue Location Ubiquitous with higher expression in the lung and vascular endothelium
Citations (0)
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Background

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).

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Discontinued
Cat# AP52036
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