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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-NOX2/gp91phox Polyclonal Antibody   

Rabbit Anti-NOX2/gp91phox Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Rabbit Anti-NOX2/gp91phox Polyclonal Antibody AP52079
    Mouse spleen lysates probed with NOX2/gp91phox Polyclonal Antibody, unconjugated (AP52079) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
  • IHC-P - Rabbit Anti-NOX2/gp91phox Polyclonal Antibody AP52079
    Formalin-fixed and paraffin embedded rat pancreas labeled with Anti NOX2/gp91phox Polyclonal Antibody, Unconjugated (AP52079) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • IHC-P - Rabbit Anti-NOX2/gp91phox Polyclonal Antibody AP52079
    Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-NOX2/gp91phox Polyclonal Antibody, Unconjugated (AP52079) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • IHC-P - Rabbit Anti-NOX2/gp91phox Polyclonal Antibody AP52079
    Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-NOX2/gp91phox Polyclonal Antibody, Unconjugated (AP52079) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P04839
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 65336 Da
Additional Information
Gene ID 1536
Other Names CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; p22 phagocyte B-cytochrome; CYBB
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CYBB
Synonyms NOX2
Function Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Detected in neutrophils (at protein level).
Citations (0)

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Background

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.

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$ 295.00
Cat# AP52079
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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