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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody   

Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody AP52080
    Lane 1: rat brain lysates Lane 2:rat heart lysates probed with Anti P45017A1/Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (AP52080) at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted band 56kD. Observed band size: 56kD.
    detail
  • WB - Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody AP52080
    Lane 1:rat brain lysates probed with Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (AP52080) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:5000 for 90 min at 37˚C.
    detail
  • WB - Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody AP52080
    Mouse Testis lysates probed with Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (AP52080) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
    detail
  • IHC-P - Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody AP52080
    Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti P45017A1 Polyclonal Antibody,Unconjugated (AP52080) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P
Primary Accession P05093
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 57371 Da
Additional Information
Gene ID 1586
Other Names CPT7; CYP17; S17AH; P450C17; Steroid 17-alpha-hydroxylase/17, 20 lyase; 17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase; CYP17A1
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CYP17A1 {ECO:0000303|PubMed:19793597, ECO:0000312|HGNC:HGNC:2593}
Function A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938). Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (PubMed:9452426, PubMed:25301938) (Probable). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938, PubMed:36640554). Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA (PubMed:36640554). Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed:27339894, PubMed:25301938). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938).
Cellular Location Endoplasmic reticulum membrane. Microsome membrane
Citations (0)
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Background

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

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Discontinued
Cat# AP52080
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