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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-ICAM1 Polyclonal Antibody   

Rabbit Anti-ICAM1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Rabbit Anti-ICAM1 Polyclonal Antibody AP52125
    Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54/ICAM-1 Polyclonal Antibody, Unconjugated (AP52125) at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted band 60kD. Observed band size: 60kD.
  • IHC-P - Rabbit Anti-ICAM1 Polyclonal Antibody AP52125
    Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (AP52125), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
  • IF - Rabbit Anti-ICAM1 Polyclonal Antibody AP52125
    Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody AP52125, Unconjugated at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C and DAPI staining
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P13597
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 58844 Da
Additional Information
Gene ID 15894
Other Names CD54; Ly-47; Icam-1; MALA-2; Intercellular adhesion molecule 1; MyD1; Icam1
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
IF~~1:100~1:200
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name Icam1
Synonyms Icam-1
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells
Citations (0)

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Background

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

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$ 295.00
Cat# AP52125
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
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