|Application ||WB, IHC-P, E|
|Other Accession||B0BND0, Q58D68, Q8BGN3|
|Calculated MW||50241 Da|
|Antigen Region||170-197 aa|
|Other Names||Ectonucleotide pyrophosphatase/phosphodiesterase family member 6, E-NPP 6, NPP-6, 314-, Choline-specific glycerophosphodiester phosphodiesterase, Glycerophosphocholine cholinephosphodiesterase, GPC-Cpde, ENPP6|
|Target/Specificity||This ENPP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-197 amino acids from the Central region of human ENPP6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ENPP6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'- monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor. Note=A small amount of the protein may be found in the extracellular milieu|
|Tissue Location||Predominantly expressed in kidney and brain. In the kidney, expressed specifically in the proximal tubules and thin descending limbs of Henle (at protein level)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
ENPP6 is choline-specific glycerophosphodiester phosphodiesterase. This protein hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate.
Sakagami, H., et al. J. Biol. Chem. 280(24):23084-23093(2005)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.