|Application ||WB, IHC-P, E|
|Other Accession||B0BND0, Q58D68, Q8BGN3|
|Calculated MW||50241 Da|
|Antigen Region||170-197 aa|
|Other Names||Ectonucleotide pyrophosphatase/phosphodiesterase family member 6, E-NPP 6, NPP-6, 314-, Choline-specific glycerophosphodiester phosphodiesterase, Glycerophosphocholine cholinephosphodiesterase, GPC-Cpde, ENPP6|
|Target/Specificity||This ENPP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-197 amino acids from the Central region of human ENPP6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ENPP6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'- monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor. Note=A small amount of the protein may be found in the extracellular milieu|
|Tissue Location||Predominantly expressed in kidney and brain. In the kidney, expressed specifically in the proximal tubules and thin descending limbs of Henle (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
ENPP6 is choline-specific glycerophosphodiester phosphodiesterase. This protein hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate.
Sakagami, H., et al. J. Biol. Chem. 280(24):23084-23093(2005)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
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