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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-Cyclin E Polyclonal Antibody   

Rabbit Anti-Cyclin E Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Rabbit Anti-Cyclin E Polyclonal Antibody AP52202
    Hela cells lysate probed with Anti-Cyclin E Polyclonal Antibody (AP52202) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody at 1:5000 90min in 37˚C.
  • IHC-P - Rabbit Anti-Cyclin E Polyclonal Antibody AP52202
    Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Rabbit Anti-Cyclin E Polyclonal Antibody, Unconjugated (AP52202) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P39949
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 47482 Da
Additional Information
Other Names Ccne; CYCLE; G1/S-specific cyclin-E1; Ccne1
Dilution WB~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name Ccne1
Synonyms Ccne
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Cellular Location Nucleus.
Citations (0)

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The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. Transcript Variant: This variant (1) contains a different 5' end region, which includes an upstream in-frame translation start codon, when compared to variant 2. The encoded protein has a 15 aa longer N-terminus, as compared to isoform 2.

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$ 295.00
Cat# AP52202
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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