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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-CD163/M130 Polyclonal Antibody   

Rabbit Anti-CD163/M130 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - Rabbit Anti-CD163/M130 Polyclonal Antibody AP52291
    Mouse splenocytes probed with Rabbit Anti-CD163/M130 Polyclonal Antibody, FITC Conjugated (AP52291-FITC) at 1:10 for 30 minutes compared to control unstained cells (blue) and isotype control (orange).
  • FC - Rabbit Anti-CD163/M130 Polyclonal Antibody AP52291
    RSC96 cells probed with CD163/M130 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (AP52291-A647) at 1:20 for 30 minutes compared to control cells (blue)and isotype control (orange).
  • WB - Rabbit Anti-CD163/M130 Polyclonal Antibody AP52291
    L1 rat brain, L2 mouse uterus lysates probed (AP52291) at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted and observed band size: 130kDa.
  • IHC-P - Rabbit Anti-CD163/M130 Polyclonal Antibody AP52291
    Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163/M130 Polyclonal Antibody, Unconjugated (AP52291) followed by conjugation to the secondary antibody and DAB staining
  • IHC-P - Rabbit Anti-CD163/M130 Polyclonal Antibody AP52291
    Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CD163 Polyclonal Antibody, Unconjugated AP52291 at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • IHC-P - Rabbit Anti-CD163/M130 Polyclonal Antibody AP52291
    Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated AP52291 at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession Q86VB7
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 125451 Da
Additional Information
Gene ID 9332
Other Names M13; MM13; Scavenger receptor cysteine-rich type 1 protein M13; Hemoglobin scavenger receptor; CD163
Dilution FC~~1:20~1:100
WB~~1:100~1:500
IHC-P~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CD163
Synonyms M130
Function Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
Cellular Location Soluble CD163: Secreted
Tissue Location Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
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Background

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

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$ 295.00
Cat# AP52291
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
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