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HAUS5 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HAUS5  Antibody (N-term) AP5230a
    Anti-HAUS5 Antibody (N-term) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - HAUS5  Antibody (N-term) AP5230a
    HAUS5 Antibody (N-term) (Cat. #AP5230a) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HAUS5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - HAUS5  Antibody (N-term) AP5230a
    HAUS5 Antibody (N-term) (Cat. #AP5230a) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O94927
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 71682 Da
Antigen Region 139-167 aa
Additional Information
Gene ID 23354
Other Names HAUS augmin-like complex subunit 5, HAUS5, KIAA0841
Target/Specificity This HAUS5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-167 amino acids from the N-terminal region of human HAUS5.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHAUS5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HAUS5
Synonyms KIAA0841
Function Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Localizes to interphase centrosomes and to mitotic spindle microtubules.
Research Areas
Citations (0)
citation

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Background

The function of this protein has not been specifically defined.

References

Lawo, S., et al. Curr. Biol. 19(10):816-826(2009)
Uehara, R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(17):6998-7003(2009)

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$ 365.00
$ 140.00
Cat# AP5230a
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