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RABBIT ANTI-PUMA POLYCLONAL ANTIBODY

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RABBIT ANTI-PUMA POLYCLONAL ANTIBODY AP52311
    K562 lysates probed with PUMA Polyclonal Antibody, unconjugated (AP52311) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
    detail
  • IHC-P - RABBIT ANTI-PUMA POLYCLONAL ANTIBODY AP52311
    Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (AP52311) at 1:200 followed by conjugation to the secondary antibody and DAB staining
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P
Primary Accession Q96PG8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 26498 Da
Additional Information
Gene ID 27113
Other Names JFY1; PUMA; JFY-1; Bcl-2-binding component 3; p53 up-regulated modulator of apoptosis; BBC3
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name BBC3
Synonyms PUMA
Function [Isoform 3]: Does not affect cell growth.
Cellular Location Note=Contrary to isoforms 1 and 2, isoform 3 does not localize to the mitochondria
Research Areas
Citations (0)
citation

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Background

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.

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Discontinued
Cat# AP52311
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