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eIF4B (Phospho-Ser422) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - eIF4B (Phospho-Ser422) Antibody AP52400
    Western blot analysis of extracts from NIH/3T3cells, using eIF4B (Phospho-Ser422) antibody.
  • WB - eIF4B (Phospho-Ser422) Antibody AP52400
    Western blot analysis of extracts from 3T3 cells (Lane 2), using eIF4B (Phospho-Ser422) Antibody. The lane on the left is treated with synthesized peptide.
  • IF - eIF4B (Phospho-Ser422) Antibody AP52400
    Immunofluorescence analysis of NIH/3T3 cells, using eIF4B (Phospho-Ser422) antibody.
  • IHC - eIF4B (Phospho-Ser422) Antibody AP52400
    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using eIF4B (Phospho-Ser422) antibody.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession P23588
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 69151 Da
Additional Information
Gene ID 1975
Other Names Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B
Dilution WB~~1:1000
IF~~1:100
IHC~~1:50~100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name EIF4B
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Research Areas
Citations (0)

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Background

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

References

Milburn S.C.,et al.EMBO J. 9:2783-2790(1990).
Yokoyama K.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
Cat# AP52400
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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